Nicolaus Avenue is a street that leads between the intersection of Highway 99 and Garden Highway in Nicolaus and 9th Street in Lincoln. It also passes through East Nicolaus and Trowbridge. It is interrupted slightly at Pleasant Grove Road, which it intersects slightly farther north from the west side of Pleasant Grove Road than from the east side of Pleasant Grove Road. East of Brewer Road, it is known as Nicolaus Road.

Nicolaus Avenue and the town of Nicolaus are named for Nicolaus Allgeier. From west to east, significant places on Nicolaus Avenue include East Nicolaus Market, Church Trucking, and East Nicolaus High School.